兔抗C4d 抗体-FITC 背景简介：
C4d是抗体触发补体通过经典途径活化后C4的裂解产物，能稳定地结合在发生排斥的移植物内皮的表面。检测C4d作为机体针对同种异体移植物抗体检测的一个间接信号，大量研究表明：C4d是肾移植免疫中的重要标记物，同时也是心脏、肝免疫检测的重要标记物。用于流式或发光法检测石蜡及冰冻切片C4d补体。 检测原理： 包被了HLA抗原的微球与被检血清中的抗HLA抗体结合（A），形成抗原抗体复合物。加入补体后（血清取自健康非敏感人群），在微球表面激活了典型的补体级联反应（B）。用c检测结合的C4d。产品优势： 用于检测人C4d补体。 该抗体可用于发光法或用One Lambda 公司的LABScreen® Single Antigen beads流式检测细胞内或固相结合的C4或C4d片段。
试剂特点： 检测样本：血清 来源：兔多克隆抗体 形式：50ul,PE 标记纯化的IgG片段。
4）应用：Luminex®-检测、流式检测 参考文献： 1 | C4d deposition is associated with inferior kidney allograft outcome independ.of cellular rejection. Regele H. et al., Nephrol Dial Transplant. 2001, 16(10): 2058-2066. | 2 | Capillary C4d deposition in kidney allografts: a specific marker of alloantibody-dependent graft injury. Böhmig GA et al., J Am Soc Nephrol. 2002, 13(4): 1091-1099. | 3 | [C4d]FlowPRA screening—a specific assay for selective detection of complement-activating anti-HLA alloantibodies. Wahrmann M et al., Hum Immunol, 2005, 66(5): 526-534 | 4 | Pivotal role of complement-fixing HLA alloantibodies in presensitized kidney allograft recipients. Wahrmann M et al., Am J Transplant, 2006, 6: 1033-1041. | 5 | In vitro detection of C4d-fixing HLA alloantibodies: associations with capillary C4d deposition in kidney allografts. Bar G et al., Am J Transplant, 2008, 8(1): 41-49 | 6 | Antibodies, isotypes and complement in allograft rejection. Böhmig GA et al., Curr Opin Organ Transplant, 2008, 13(4): 411-418 | 7 | Solid phase detection of C4d-fixing HLA antibodies to predict rejection in high immunological risk kidney transplant recipients. Bar G et al., Transpl Int. 2013; 26(2): 121-130. | 8 | Preformed complement-activating low-level donor-specific antibody predicts early antibody-mediated rejection in renal allografts. Lawrence C et al., Transplantation 2013 Jan 27; 95(2): 341-346. | 9 | Modified solid-phase alloantibody detection for improved crossmatch prediction. Wahrmann M et al., Hum Immunol. 2013 Jan; 74(1): 32-40. |
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