SpeciesReactivity
Rat Form
Stds=2.5-60 ng/ml ; Sample=100 ul (diluted); Sensitivity= 2 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests Samples
Serum. Rat CRP assay in Plasma (EDTA), culture medium (VSMC cells) have also been used (see published references). Other fluids may be adapted KitContents
1. Rabbit Anti-Rat CRP IgG coated strip plate ( 8 wells x 12 strips) 1011 2. Rat CRP Std. A (0 ng/ml) 0.2 ml 1012 3. Rat CRP Std. B (12.5 ng/ml) 0.2 ml 1013 4. Rat CRP Std. C (25 ng/ml) 0.2 ml 1014 5. Rat CRP Std. D (100 ng/ml) 0.2 ml 1015 6. Rat CRP Std. E (300 ng/ml) 0.2 ml 1016 7. 100x Anti-rat CRP-HRP Conjugate, 0.12 ml 1017 8. 100x Wash Buffer, 10 ml WB-100 9. 20x Sample Diluent, 10ml SD-20 10. TMB Substrate, 12 ml 80091 11. Stop solution, 12 ml 80101 12. Instruction Manual M-1010 SpCrossreactivity
Serum samples from mouse, human, pig, goat, FBS, Bovine, G. pig, hamster, monkey, and Chicken showed less than 1% crossreactivity with rat CRP ELISA kit at dilutions of 1:400. Rabbit serum showed ~10% cross reactivity. ADI provides human CRP ELISA kit cat # 1000 for detecting human serum CRP. ELISA kits for mouse, rat, human, monkey, rabbit CRP are also available. Rat IgG, IgM, and other serum protein ELISA kits can be obtained from ADI. MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use. |
