猪I型胶原（3D凝胶制备） Type I Collagen

产品名称

可溶性I型胶原

应用

用于传统的二维（2D）系统以及三维（3D）凝胶中的支架，用于模拟成纤维细胞和软骨细胞中的细胞生长。

规格

4 mg/ml x 12.5 ml (sterile filtered)

形态

溶于0.01M HCL

来源

猪

内毒素

＜1 EU/ml

纯度

＞95% I 胶原

保存

4℃

稳定性

6月

操作流程

包被（注：具体的包被的条件需要根据培养系统优化）

1.用0.02mol/L的盐酸稀释4mg/ml的胶原到50-100ug/ml。

2.温和混匀稀释后的溶液。

3.取适量的稀释后的胶原加入孔内或板内。

注：保证整个表面都是包被的。

4. 37℃或室温孵育1-2小时

5. 弃掉所有溶液。

6.用培养基或者PBS漂洗包被表面。

7.包被好的孔或板2-8℃保存或者空气中晾干（如果保持无菌）

三维凝胶制备流程：

1. 用PBS等体积稀释胶原溶液，终浓度2mg/ml。
2. 加入适量的稀释后的胶原到孔内或板内。
3. 37℃孵育30~60分钟。
4. 凝胶可以2-8℃保存或者空气中晾干（如果保持无菌）。

参考文献

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